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KMID : 0545120060160121935
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Journal of Microbiology and Biotechnology
2006 Volume.16 No. 12 p.1935 ~ p.1939
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Cloning and Analysis of Medium-Chain-Length Poly(3-Hydroxyalkanoate) Depolymerase Gene of Pseudomonas luteola M13-4
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Park In-Jae
Rhee Young-Ha
Cho Nam-Young
Shin Kwang-Soo
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Abstract
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The gene encoding the extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA) depolymerase of Pseudomonas luteola Ml3-4, , was cloned and analyzed. It was found to be 849 bp, with a deduced protein of 282 amino acids, and was revealed to have a typical leader peptide at its N terminus. The amino acid sequence of revealed relatively low identity (69 to 72%) with those of other Pseudomonas MCL-PHA depolymerases. In comparison with the amino acid sequences of all available MCL-PHA depolymerases, the depolymerase was found to consist of three domains in sequential order; signal peptide, an N-terminal substrate binding domain, and a catalytic domain, indicating that belongs to the type IV depolymerases family. The enzyme also contained Asn as an oxyanion hole amino acid.
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KEYWORD
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Molecular analysis, MCL-PHA depolymerase gene, Pseudomonas luteola M13-4
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